Alterations in the Secretome of Clinically Relevant Preparations of Adipose-Derived Mesenchymal Stem Cells Cocultured with Hyaluronan

Figure 2

Morphology and differentiation potential of control and treated MSCs. MSCs cultured in either standard culture media or HA media. No detectable differences were seen in the morphology of control (a) MSCs and HA treated (f) monolayers. Osteogenic differentiation of control MSCs (b) and of the HA treated (g) at 10x magnification. Adipogenic differentiation of control (c) and of the HA treated (h) at 10x magnification. At 20x magnification no detectable differences in Alizarin Red S staining were observed in the osteogenic differentiation of control MSCs (d) and HA treated (i). At 20x magnification no detectable differences in Oil Red O staining were observed in the adipogenic differentiation of control MSCs (e) and HA treated (j).