Alterations in the Secretome of Clinically Relevant Preparations of Adipose-Derived Mesenchymal Stem Cells Cocultured with Hyaluronan

Figure 6

Fold change in concentration of growth factors detected across the experiments. Average fold change in concentration () ± upper and lower confidence intervals at 95% (-axis). Conditions (-axis): stromal vascular fraction on day 1 (SVF D1), stromal vascular fraction on day 3 (SVF D3), stromal vascular fraction plus mature adipocytes on day 1 (SVF + adipo D1), stromal vascular fraction plus mature adipocytes on day 3 (SVF + adipo D3), seeding experiment with purified MSCs (MSC seeding), and media change experiment with purified MSCs (MSC media). Conditions were all cultured in either standard culture media or HA media. Cytokines with a fold change less than one indicate a decrease in concentration with HA media treatment, and a fold change greater than one indicates an increase in the cytokine with HA treatment. Hepatocyte growth factor (HGF), fibroblast growth factor-basic (FGF-β), nerve growth factor-beta (β-NGF), granulocyte macrophage colony-stimulating factor (GM-CSF), vascular endothelial growth factor (VEGF), and granulocyte colony-stimulating factor (G-CSF). GM-CSF was not detected across all biological replicates in the MSC media condition.