Alterations in the Secretome of Clinically Relevant Preparations of Adipose-Derived Mesenchymal Stem Cells Cocultured with Hyaluronan

Figure 7

Fold change in concentration of chemokines detected across the experiments. Average fold change in concentration () ± upper and lower confidence intervals at 95% (-axis). Conditions (-axis): stromal vascular fraction on day 1 (SVF D1), stromal vascular fraction on day 3 (SVF D3), stromal vascular fraction plus mature adipocytes on day 1 (SVF + adipo D1), stromal vascular fraction plus mature adipocytes on day 3 (SVF + adipo D3), seeding experiment with purified MSCs (MSC seeding), and media change experiment with purified MSCs (MSC media). Conditions were all cultured in either standard culture media or HA media. Cytokines with a fold change less than one indicate a decrease in concentration with HA media treatment, and a fold change greater than one indicates an increase in the cytokine with HA treatment. Chemokine ligand-1 (CXCL-1) aka growth-regulated peptide alpha (GROα), CC-Chemokine ligand-4 (CCL-4) aka macrophage inflammatory protein-1 beta (MIP-1β), CC-Chemokine ligand-5 (CCL-5) aka regulated on activation normal T cell expressed and secreted (RANTES), CC-chemokine ligand-2 (CCL-2) aka monocyte chemotactic protein-1 (MCP-1), CC-chemokine ligand-7 (CCL-7) aka monocyte chemotactic protein-3 (MCP-3), and CC-chemokine ligand-3 (CCL-3) aka macrophage inflammatory protein-1-alpha (MIP-1α). MCP-3 was not detected across all biological replicates in the MSC media condition. MCP-1 was only detected in one of three biological replicates in the SVF + adipo D3 condition.