Differential Reponses of Hematopoietic Stem and Progenitor Cells to mTOR Inhibition

Figure 1

Inhibition of mTOR depletes HSPCs in culture. (a) Phosphorylated S6K (pS6K) was determined using a pS6K specific monoclonal antibody and immunofluorescence microscopy. (b) The percentage of pS6K positive cells was calculated and is presented as mean +/− SEM. (c) The frequency of Lin⁻Sca-1⁺c-Kit⁺ (LSK⁺) cells (HSCs/MPPs enriched subpopulations) and Lin⁻Sca-1⁻c-Kit⁺ (LSK⁻) cells (HPCs) in cultures after AZD treatment was determined by flow cytometric analyses and representative flow cytometric graphs are presented. (d) The frequency of LSK⁺ cells in cultured mouse BM Lin⁻ cells at 20 h after AZD treatment is presented as mean +/− SEM. (e) The frequency of HPCs in in cultured mouse BM Lin⁻ cells at 20 h after AZD treatment is presented. Data are presented as mean +/− SEM of three independent experiments.; versus DMSO vehicle control.

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