Differential Reponses of Hematopoietic Stem and Progenitor Cells to mTOR Inhibition

Figure 2

Inhibition of mTOR induces apoptosis in HSPCs. (a) Representative flow cytometric graphs of apoptosis assays using an apoptosis analysis kit as we previously reported [13]. (b) The percentage of apoptotic cells in LSK⁺ subpopulations after different doses of AZD treatment is presented. (c) The percentage of apoptotic cells in LSK⁻ subpopulations after different doses of AZD treatment is presented. (d) The percentage of apoptotic LSK⁺ cells in cultured mouse BM Lin⁻ cells after KU-63794 (10 M) treatment is presented. (e) Mouse lineage negative HSPCs were treated with AZD (0.1 M) or DMSO as vehicle control for 16 h. Active caspase-3 immunostaining was performed to determine apoptotic cells in HSPCs. Representative photomicrographs of active caspase-3 immunofluorescent staining (red) and nucleic counterstaining with DAPI (blue) are shown. Data are presented as mean +/− SEM of three independent experiments. ; versus DMSO vehicle control.

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