Differential Reponses of Hematopoietic Stem and Progenitor Cells to mTOR Inhibition

Figure 4

Dual inhibition of mTORC1/2 suppresses day-14 CAFCs but promotes the production of day-35 CAFCs. The functions of HSPCs were determined in vitro using CAFC assays as we previously reported [13–15]. (a) Day-14 CAFCs were analyzed to assess the function of HPCs. (b) Day-35 CAFCs were measured to evaluate the function of HSCs in vitro. (c) Day-35 CAFCs were determined using the same protocol as shown in (b), except that KU-63974 was readded to the culture medium weekly for 4 weeks. (d) Day-14 CAFCs were performed to assess the function of HPCs in the presence of different doses of AZD. (e) Day-35 CAFCs were measured to evaluate the function of HSCs with or without AZD treatment. Data are presented as mean +/− SEM of three independent experiments. versus DMSO vehicle control; ; versus DMSO vehicle control.

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