What Makes Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Superior Immunomodulators When Compared to Bone Marrow Derived Mesenchymal Stromal Cells?

Figure 3

UCX are more potent immunosuppressors than BM-MSCs. (a) Activation of PBMCs from two different donors was induced by incubation with anti-CD3, anti-CD28, and IL-2 (control). Suppression of lymphocyte proliferation was analysed in the presence of irradiated non-MSCs (Molt4), BM-MSCs, and UCX (both naïve and primed with 10 ng/mL of TNFα and IFNγ). Independent of donor, lymphocyte proliferation was significantly suppressed when cells were cocultured with MSCs, but not with non-MSCs. Values are represented as mean s.e.m. and statistically significant differences are indicated by asterisks (nonparametric -test Mann Whitney, ). (b) Treg conversion was assayed by using FACS-sorted CD3⁺CD4⁺CD25⁻ T cells that were activated with anti-CD3, anti-CD28, and IL-2 alone and with the addition of TGF-. The effect of irradiated UCX and BM-MSCs in T cell conversion was analysed in cultures without exogenous TGF- by flow cytometry analysis of CD25 and Foxp3 expression. Values are represented as mean s.e.m. and statistically significant differences are indicated by asterisks (unpaired -test, ).

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