A Double-Switch Cell Fusion-Inducible Transgene Expression System for Neural Stem Cell-Based Antiglioma Gene Therapy

Figure 4

DoFIT mediates NSC-delivered glioma-specific transgene expression in vivo. (a) Bioluminescent images showing transgene expression in the glioma-bearing mice. U251 cells are inoculated into the right forebrains of the mice. After the gliomas develop, DoFIT-NSCs are inoculated into both the left and the right forebrains of the mice. Bioluminescent images are taken on day 1 and day 2 after NSC inoculation. The luminescent readings (photons/sec) of both left and right forebrains are indicated below the images. (b) Line graph showing trends of the averaged transgene expression levels in the glioma sites (right forebrain) and the normal sites (left forebrain) over day 1 and day 2 after NSC inoculation. Statistical significance of glioma sites versus normal sites at each time point was calculated by Student’s -test. Error bars: s.d. .

(a)

(b)