Targeted Knockdown of RNA-Binding Protein TIAR for Promoting Self-Renewal and Attenuating Differentiation of Mouse Embryonic Stem Cells

Table 3

Primers used for reverse-transcription polymerase chain reaction.

Gene	Sense primer (5′-3′)	Antisense primer (5′-3′)	Annealing temperature (°C)	Size (bp)
Oct4	GCTCAGCCTTAAGAACATGTGTAAGC	GCCTCATACTCTTCTCGTTGGGA	56	327
Nanog	ATGAAGTGCAAGCGGTGGCAGAAA	CCTGGTGGAGTCACAGAGTAGTTC	56	464
Sox2	GGCGGCAACCAGAAGAACAG	GTTGCTCCAGCCGTTCATGTG	56	414
Klf4	CAGTCGCAAGTCCCCTCTCTC	CCTGTCGCACTTCTGGCACTG	56	321
GAPDH	ACTCACGGCAAATTCAACGG	ACGTCAGATCCACGACGGAC	56	586