Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro

Table 1

Primer sequences in qPCR assay.

Primer	Sequence (5′ to 3′)	Product size (bp)	Gene accession number
TIMP-1-F	CCTTTGCATCTCTGGCATCT	141	NM_001044384
TIMP-1-R	GGGAACCCATGAATTTAGCC
MMP-9-F	TCCGTGTCCTGTAAATCTGCT	152	NM_013599
MMP-9-R	GACCTGAACCATAACGCACA
MMP-2-F	TTCAACGGTCGGGAATACAG	103	NM_008610
MMP-2-R	AGCCATACTTGCCATCCTTC
TNF-α-F	CACCACCATCAAGGACTCAA	102	NM_001278601
TNF-α-R	GAGACAGAGGCAACCTGACC
bFGF-F	AGGAAGATGGACGGCTGCT	138	NM_008006
bFGF-R	GCCCAGTTCGTTTCAGTGC
HIF-1α-F	TGAACATCAAGTCAGCAACG	129	NM_010431
HIF-1α-R	CACAAATCAGCACCAAGCAC
SDF-1-F	TCTTAGGAGGCACAGCAAGC	101	NM_001012477
SDF-1-R	CAGGCATTTCCATGAGGACT
G-CSF-F	TGCACTATGGTCAGGACGAG	108	NM_009971
G-CSF-R	CTCACTTGCTCCAGGGACTT
EGF-F	GCTCTTCTGGGTTCAGGACA	112	NM_010113
EGF-R	AGACAAACTGTGCCGTGCTT
HGF-F	TGCTCCTCCCTTCCCTACTC	109	NM_001289458
HGF-R	CGGGCTGAAAGAATCAAAGC
VEGF-F	CTGCTGTGGACTTGTGTTGG	106	NM_001025250
VEGF-R	AAAGGACTTCGGCCTCTCTC
MCP-1-F	CCCACTCACCTGCTGCTACT	101	NM_011333
MCP-1-R	CAGCTTCTTTGGGACACCTG
GAPDH-F	GGAGCCAAACGGGTCATCATCTC	121	NM_008084
GAPDH-R	AGTGGGAGTTGCTGTTGAAGTCGC