In Vitro Differentiation Potential of Human Placenta Derived Cells into Skin Cells

Table 2

List of reagents and antibodies.

Sr. number	Reagents/antibodies	Details
1	Phosphate buffered saline	Sigma Aldrich, USA
2	Fetal calf serum	Sigma Aldrich, USA
3	Penicillin and streptomycin	Sigma Aldrich, USA
4	DMEM	Sigma Aldrich, USA
5	0.25% trypsin-EDTA	Sigma Aldrich, USA
6	Crystal violet dye	Sigma Aldrich, USA
7	Fetal bovine serum	Sigma Aldrich, USA
8	HAM F12	Sigma Aldrich, USA
9	Hydrocortisone	Sigma Aldrich, USA
10	Insulin transferrin	Sigma Aldrich, USA
11	DAPI	Sigma Aldrich, USA
12	CK5 antibody	Sigma Aldrich, USA
13	CK10 antibody	Sigma Aldrich, USA
14	Involucrin antibody	Sigma Aldrich, USA
15	Insulin	Sigma Aldrich, USA
16	Basic fibroblast growth factor	Sigma Aldrich, USA
17	Trizol reagent	Sigma Aldrich, USA
18	Reverse transcriptase kit	Invitrogen, USA
19	Keratinocytes growth factor	Invitrogen, USA
20	CD45 antibody	Abcam, UK
21	CK8 antibody	Abcam, UK
22	CK18 antibody	Abcam, UK
23	CK19 antibody	Abcam, UK
24	CD49 antibody	Abcam, UK
25	CD73 antibody	Abcam, UK
26	CD90 antibody	Abcam, UK
27	FAP-α antibody	Abcam, UK
28	Loricrin antibody	Abcam, UK
29	Collagen-3 antibody	Santa Cruz Biotechnology, USA
30	Desmin antibody	Santa Cruz Biotechnology, USA
31	E-Cadherin	Santa Cruz Biotechnology, USA
32	Procollagen-1 antibody	Santa Cruz Biotechnology, USA