CCR2 Positive Exosome Released by Mesenchymal Stem Cells Suppresses Macrophage Functions and Alleviates Ischemia/Reperfusion-Induced Renal Injury

Figure 3

CCR2-positive MSC-derived exosomes suppress macrophage migration and activation. (a) Transwell assay assessing the migration ability of mouse macrophages. The migrated cells on the membranes were counted under the microscope and both the representative figures and averaged cell counts for each group were shown. (b)-(c) Raw264.7 cells were stimulated with CCL2 in the presence of exosomes from the MSCs or NIH3T3 cells. Then, the quantitative RT-PCR analysis was used for the mRNA levels of inflammation factors TNFA, IL6, and IL1B in the macrophages (b). The change folders for each group compared to the CCL2 alone treated macrophages were shown. The WB analysis was used to determine the phosphorylation levels of NF-κB p65 (p-p65) with GAPDH as an internal reference protein. The gray levels of blots were analyzed and the ratios of p-p65/GAPDH were shown (c). Exos: exosomes; si-CCR2 MSC-exo: exosomes derived from CCR2 siRNAs-transfected MSCs; NC RNA MSC-exo: exosomes derived from negative control RNA-transfected MSCs; bars represent standard error of the mean ± SD from three repeats. All the significance was determined by ANOVA and Student’s -test. .

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