CCR2 Positive Exosome Released by Mesenchymal Stem Cells Suppresses Macrophage Functions and Alleviates Ischemia/Reperfusion-Induced Renal Injury

Figure 4

CCR2-positive MSC-derived exosomes reduce ischemia/reperfusion-induced renal injury. Babl/c mice were anesthetized and the ischemia/reperfusion operation was performed by clamping the renal vessels for 60 mins. A sham operation was also performed without renal vessels clamping. At 10 min after the surgery, mice received a renal capsule injection of 200 μg of exosomes per kidney or only PBS as a control. (a)-(b) Blood urea nitrogen (BUN) and creatinine (Cr) values from 0 days to 5 days. Bars represent standard error of the mean ± SD from three repeats. (c)-(d) Representative micrographs of renal histology at day 5 were shown (c). Scale bars, 100 μm. Morphologic findings were also scored according to cast deposition (cast), tubular dilation (dilation), tubular degeneration (degeneration), and tubular necrosis (necrosis), and the average scores for each group were shown as mean ± SD from six repeats (d). (e)-(f) Representative micrographs of F4/80 staining of mice kidney section at day 5 were shown (e), scale bars, 50 μm. The counts of positive cells per field were also shown as the mean ± SD from six repeats (f). All the significance was determined by ANOVA and Student’s -test. . Exos: exosomes; si-CCR2 MSC-exo: exosomes derived from CCR2 siRNAs-transfected MSCs; NC RNA MSC-exo: exosomes derived from negative control RNA-transfected MSCs.

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