Cytokines TNF-α, IL-6, IL-17F, and IL-4 Differentially Affect Osteogenic Differentiation of Human Adipose Stem Cells

Figure 1

Comparative analysis of the effect of pro- and anti-inflammatory cytokines on hASC proliferation. ASCs were stimulated for 72 h with proinflammatory cytokines TNF-α, IL-6, IL-8, and IL-17F and the anti-inflammatory cytokine IL-4 (10 ng/mL). (a) Gene expression of proliferation marker KI67 at 6 h, 48 h, and days 4 and 7. No significant effects of cytokines on KI67 expression were found, . (b) DNA content at 48 h, day 4, and day 7. TNF-α decreased DNA content at 48 h but increased DNA content at days 4 and 7. IL-17F decreased DNA content at day 4. , results are mean SD. effect of cytokine treatment, .

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