The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation

<div>iPSCs generated using the established protocol. (a) Brightfield image of iPSCs generated from PBMCs and CBMCs. (b) Alkaline phosphatase staining results of generated iPSCs. (c) TRA-1-60 stained fluorescence image of iPSCs. (d) Karyotype results of generated iPSCs. All cell lines showed normal karyotype. (e) Ectoderm differentiation, (f) mesoderm differentiation, and (g) endoderm differentiation image of iPSCs. Scale bars: 200 <i>μ</i>m.</div>

Stem Cells International

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Figure 5

Figure 5: The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation 

Figure 5 | The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation 