Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells

Figure 4

The effect of OP9 and OP9DL1 on proliferation of mature B cells. The B cells (B220⁺) isolated from mice spleens were stained with CFSE, then exposed with 25 ng/mL IL4 plus 10 μg/mL LPS for 24 h, and subsequently cultured alone or together with OP9 or OP9DL1 cells at different rates for 36 h. At 36 h later, the morphology of B cells in each well was observed microscopically (a), then the B cells were analyzed by FACS, and proliferation was measured by the reduction in CFSE intensity. Cell growth rates are indicated by percentages in (b). Data are representative of three experiments.

(a)

(b)