In Vitro Osteogenic Potential of Green Fluorescent Protein Labelled Human Embryonic Stem Cell-Derived Osteoprogenitors

Figure 2

Characterization of hESC-OPs (GFP+) by real-time RT-PCR for pluripotency (OCT4, SOX2, and NANOG), mesenchymal (COL1A1 and COL3A1), chondrogenic (COL2A1), and osteogenic (RUNX2, OSTERIX, and OPG) lineage-associated transcripts. The transcript levels were normalized to respective β-Actin levels and to hESC (GFP+) cells. Values represent the means ± SD of three experiments ( versus hESC control).