Mesenchymal Stem Cells Derived from Human Exocrine Pancreas Spontaneously Express Pancreas Progenitor-Cell Markers in a Cell-Passage-Dependent Manner

Table 2

Primers and probes used for qPCR amplification.

Gene		Sequence ()	(°C)	Product size (bp)
Pdx1	Forward	GCATCCCAGGTCTGTCTTCT	60.7	140
	Reverse	CACTGCCAGAAAGGTTTGAA	58.5
	Probe	AACCCGAGCACAAGGCCCAG	62.9	3′TAMRA, 5′FAM
Ngn3	Forward	GAAAGGACCTGTCTGTCGCT	59	124
	Reverse	AGGGAGAAGCAGAAGGAACA	57.7
	Probe	CTCCCGGCTCCCTCCCTCTC	62.9	3′TAMRA, 5′FAM
MafA	Forward	TTCAGCAAGGAGGAGGTCAT	60.3	119
	Reverse	TTCTCGCTCTCCAGAATGTG	61.5
	Probe	CTCAAGCAGAAGCGGCGCAC	62.7	3′TAMRA, 5′FAM
B2M	Forward	GACTTTGTCACAGCCCAAGA	59.2	111
	Reverse	CAAGCAAGCAGAATTTGGAA	61.8
	Probe	TCATCCAATCCAAATGCGGCA	62.7	3′TAMRA, 5′FAM