Mesenchymal Stem Cells Loaded with p5, Derived from CDK5 Activator p35, Inhibit Calcium-Induced CDK5 Activation in Endothelial Cells

Figure 4

p5 and CM from p5-primed hADMSCs inhibited CDK5 and p53 activation by calpain in cultured BAECs. (a) The cultured BAECs were treated with p5 (lanes 5–7) or conditioned medium (CM) from p5-primed hADMSCs collected on Day 1 (lanes 8–10) for 1 hour and then further treated with 5 μM calcium ionophore A23187 and 2.5 mM CaCl₂ for 10 minutes. The 3 μg/mL p5 treatment alone (lane 1), CM from untreated hADMSCs (lane 2) and untreated BAECs (lane 3) were used as control. The p35 cleavage and the phosphorylation of CDK5 and p53 were suppressed by p5 and CM from p5-primed hADMSCs. (b) Densitometric analysis of immunoblots quantification. Data are means ± SD (error bars). Significance (denoted as ) was calculated compared to cells treated with 5 μM calcium ionophore A23187 and 2.5 mM CaCl₂ (lane 4) using Student’s -test (with < 0.05).

(a)

(b)