Phenotypic, Functional, and Safety Control at Preimplantation Phase of MSC-Based Therapy

Figure 2

(a) Comparison of WJ-MSC(M) and WJ-MSC(E) population doubling time. Up to 11th passage PDT in WJ-MSC(M) remained at a relatively stable level of 1.5–2 days. In contrast, the WJ-MSC(E) have slowed down their cell divisions at the latest 3 passages (9–11). The results are mean values of 3 experiments ± SD. ; . (b) Cell growth analysis. Only WJ-MSC(M) presents a linear growth rate. After 7 days of culture the average number of WJ-MSC(E) was significantly lower than that of WJ-MSC(M). The results are mean values of 3 experiments ± SD; ; . (c) Cell proliferation. Decrease of WJ-MSC(E) proliferation rate correlated with decreased number of Ki67 reactivity. The results are mean values of 3 experiments ± SD. (d) Percentage of senescent cells counted at 11th passage. The number of cells expressing β-galactosidase is significantly higher in WJ-MSC(E) than WJ-MSC(M). The results are presented as mean values of 3 experiments ± SD, . (e) Quantification of CFU frequency. WJ-MSC isolated by mechanical method have significantly greater ability to create colonies. The results are presented as mean values of 3 experiments ± SD; ; ; .

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