Research Article
Phenotypic, Functional, and Safety Control at Preimplantation Phase of MSC-Based Therapy
Table 2
Primers used for quantitative RT-PCR.
| | Gene | Product size | Primer sequence () |
| | CD73 | 241 bp | F: CGCAACAATGGCACAATTAC | | R: CTCGACACTTGGTGCAAAGA |
| | CD90 | 236 bp | F: CTAGTGGACCAGAGCCTTCG | | R: TGGAGTGCACACGTGTAGGT |
| | CD105 | 165 bp | F: CACTAGCCAGGTCTCGAAGG | | R: CTGAGGACCAGAAGCACCTC |
| | CD166 | 217 bp | F: CGCAATGCAACAGGAGACTA | | R: GGCTAGATCGAAGCCTGATG |
| | Vimentin | 170 bp | F: GAGAACTTTGCCGTTGAAGC | | R: TCCAGCAGCTTCCTGTAGGT |
| | Collagen | 332 bp | F: AGTGGTTACTACTGGATTGACC | | R: TTGCCAGTCTCCTCATCC |
| | Fibronectin | 386 bp | F: CTGGGATGCTCCTGCTGT | | R: CTGTTTGATCTGGACCTGCAG |
| | Nestin | 169 bp | F: TGGCTCAGAGGAAGAGTCTGA | | R: TCCCCCATTTACATGCTGTGA |
| | GFAP | 266 bp | F: GCAGAGATGATGGAGCTCAATGACC | | R: GTTTCATCCTGGAGCTTCTGCCTCA |
| | NF-200 | 160 bp | F: GAGGAACACCAAGTGGGAGA | | R: TTCTGGAAGCGAGAAAGGAA |
| | β-Tubulin III | 159 bp | F: CTCAGGGGCCTTGGACATC | | R: CAGGCAGTCGCAGTTTTCAC |
| | β-actin (ACTB) | 120 bp | F: GCCAACCGCGAGAAGATGA | | R: CATCACGATGCCAGTGGTA |
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