MicroRNAs-Proteomic Networks Characterizing Human Medulloblastoma-SLCs

Figure 2

Morphology and characteristics of hMB-SLCs. (a) hMB-SLCs were cultured for 48 h in the absence or in the presence of 8 μM RA which induces SLCs differentiation, highlighted by morphological changes and cell adhesion. (b) Western blot analysis confirmed that 48 h exposure of hMB-SLCs to 8 μM RA induced a strong reduction in the stemness markers Nanog and OCT4 while increasing the differentiation markers, β-3-Tubulin and GFAP. (c) qPCR analysis of OCT4, Nanog, KLF4, β-3-Tubulin, GFAP, and BMP4 was conducted before and after RA treatment. mRNA expression levels are indicated as fold changes with respect to hMB-SLCs. (d) Gene expression of stemness, differentiation, and proliferation markers in hMB-SLCs with respect to RA-treated cells. denotes versus hMB-SLCs, denotes versus hMB-SLCs, and denotes versus hMB-SLCs.

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