Role of Nitric Oxide, Nitric Oxide Synthase, Soluble Guanylyl Cyclase, and cGMP-Dependent Protein Kinase I in Mouse Stem Cell Cardiac Development

Figure 3

Soluble GC and NOS enzymatic activity. Soluble GC and NOS enzymatic activity evaluated by cGMP measure in mEBs at day 14 of differentiation in control condition and in the presence of 100 μM L-NMA, 100 μM SNAP, 50 μM ISN, 1 μM ODQ + 100 μM SNAP, and 1 μM ODQ + 50 μM ISN. All measures were carried out in the presence of 100 μM IBMX. and versus CTR; versus SNAP, one-way ANOVA followed by post hoc Bonferroni’s test. For method, see Table 1. Data are the mean ± s.e. of at least 3 differentiations; measures were performed in duplicate.