Role of Nitric Oxide, Nitric Oxide Synthase, Soluble Guanylyl Cyclase, and cGMP-Dependent Protein Kinase I in Mouse Stem Cell Cardiac Development

Figure 5

Percentage of beating mEBs during cardiac differentiation. The percentage of beating mEBs was calculated at different time-points of mEBs cardiac differentiation. Cells were incubated with 50 μM ISN (NO-donor) or NOS/sGC/PKG-I pathway inhibitors (resp., 100 μM L-NMA, 1 μM ODQ, or 1 μM KT5823) from day 0 to day 16. Insert: beating mEBs (percentage of the total bodies) in control condition. One-way ANOVA followed by post hoc Bonferroni’s test. , , and versus control; versus control and all other treatments; versus all other treatments. Data are the mean ± s.e. of at least 4 different differentiations.