Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application

Figure 4

Quantitative immunofluorescence (q-IF) of p63 in LSC pools. (a) Histogram shows the percent of p63+ cells with diameters ≤10 μm. LSC from aged donors showed >3% p63+ cells while younger donors had <3%. (b) Comparative histograms of p63+ cells with diameters >10 μm, the overall p63+ cells (with diameters >10 μm and ≤10 μm), and the p63-cells. (c) Representative images of the analysis. Scale bar = 10 μm. Results are presented as percent of total cells. , , and .

(a)

(b)

(c)