Research Article
Limbal Stem Cells from Aged Donors Are a Suitable Source for Clinical Application
Table 1
Primary and secondary antibodies.
| | Antibody | Source | Isotype | Clone | Application | Dilution |
| | Connexin 43 | Santa Cruz Lab | Rabbit IgG | H-150 | WB | 1/1000 | | Cytokeratin 12 | Abcam | Rabbit IgG | EPR1609(2) | WB | 1/2000 | | Cytokeratin 12 | Santa Cruz Lab | Goat IgG | L-20 | IFI | 1/100 | | Cytokeratin 19 | Boehringer Mannheim | Mouse IgG1 | 170.2.14 | WB, IFI | 1/1000, 1/50 | | Cytokeratin 3 | Millipore | Mouse IgG1 | AE5 | WB, IFI | 1/2500, 1/100 | | E-cadherin | BD Transduction Laboratories | Mouse IgG2a | 36 | WB | 1/2000 | | p63 | Millipore | Mouse IgG2a | 4A4 | WB | 1/1250 | | p63 | Abcam | Mouse IgG2a | BC4A4 | IFI | 1/50 | | Tubulin | Sigma | Mouse IgG1 | DM1A | WB | 1/2000 | | Phalloidin-TRITC | Sigma | | | IFI | 1/2000 | | Goat anti-rabbit | Sigma | Goat | | WB | 1/2500 | | Rabbit anti-mouse | Sigma | Rabbit | | WB | 1/2500 | | Goat A488 | Invitrogen Life Technologies | Goat | | IFI | 1/1000 | | Mouse A488 | Invitrogen Life Technologies | Mouse | | IFI | 1/1000 |
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WB: Western blot; IFI: indirect immunofluorescence.
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