Establishment and Characterization of a Human Small Cell Osteosarcoma Cancer Stem Cell Line: A New Possible In Vitro Model for Discovering Small Cell Osteosarcoma Biology

Figure 11

Soft agar assay on OSA3, OSA3-CSCs, Saos-2, and PA. After 24 h from the setup of the assay cells appear isolated into the agar (a). Observation in phase contrast. Original magnification 10x. While after 4 weeks spherical large colonies in OSA3-CSCs and in Saos-2 (b and d) were formed into the agar, they are absent for OSA3 (c) and PA (e). Observation in phase contrast. Original magnification 20x. Bar size: 100 µm.

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