Establishment and Characterization of a Human Small Cell Osteosarcoma Cancer Stem Cell Line: A New Possible In Vitro Model for Discovering Small Cell Osteosarcoma Biology

Figure 13

Sphere formation assay on OSA3-CSCs at 14 days. After 14 days from the start of the assay, several small spherical colonies surrounded by single cells (single cells are indicated by the black/green arrows) can be observed. The sarcospheres (some of these are indicated by the black/red arrows) appear floating in the medium observation in phase contrast. Original magnification: 20x. Bar size: 100 µm.