Establishment and Characterization of a Human Small Cell Osteosarcoma Cancer Stem Cell Line: A New Possible In Vitro Model for Discovering Small Cell Osteosarcoma Biology

Figure 19

Osteogenic differentiation on OSA3-CSCs and on OSA3-CSCs II lines_HA. Osteogenic differentiation at 0 days (a-b), after 14 days (c-d), and after 21 days of induction by a cytochemical staining for the hydroxyapatite (HA) with Alizarin Red S. The cells are only contrasted in blue/grey and the grainy deposits of HA are stained in orange/red. Observation in phase contrast. Original magnifications: 20x. Bar size: 100 µm.

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