Establishment and Characterization of a Human Small Cell Osteosarcoma Cancer Stem Cell Line: A New Possible In Vitro Model for Discovering Small Cell Osteosarcoma Biology

Figure 27

Immunofluorescence staining of ESCs markers. Immunofluorescence staining of Nanog (a), POU5F1 (c), SOX2 (e), KLF4 (g), and LIN-28A (i) of the cell line of OSA3-CSCs. (b, d, f, h, and j) Negative controls, respectively, for each marker. LSCM in conventional colors: green for ESCs and pink for nuclei. Original magnifications: 20x. Bar size: 100 µm.

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