Establishment and Characterization of a Human Small Cell Osteosarcoma Cancer Stem Cell Line: A New Possible In Vitro Model for Discovering Small Cell Osteosarcoma Biology

Figure 7

Sarcosphere assay on OSA3 at 14 and at 28 days. (a) After 14 days from the start of the assay, several small spherical colonies can be observed. The sarcospheres (indicated by the yellow arrows) appear floating in the medium or slightly settled down into the bottom of the well. (b) After 28 days big spherical colonies can be observed. Observation in phase contrast. Original magnification: 20x. Bar size: 100 µm.

(a)

(b)