Establishment and Characterization of a Human Small Cell Osteosarcoma Cancer Stem Cell Line: A New Possible In Vitro Model for Discovering Small Cell Osteosarcoma Biology

Figure 8

Sarcosphere after isolation. (a) Sarcospheres from OSA3 cell lines showed a beginning of adherent expansion by reintroduction and reculturing in monolayer, adherent conditions at 48 hours from the isolation. (b) At 7 days after the isolation sarcospheres showed adherent expansion by reintroduction and reculturing in monolayer, adherent conditions. Observation in phase contrast. Original magnification: 20x. Bar size: 100 µm.

(a)

(b)