Establishment and Characterization of a Human Small Cell Osteosarcoma Cancer Stem Cell Line: A New Possible In Vitro Model for Discovering Small Cell Osteosarcoma Biology

Table 1

Detailed list of primer sequences, with the amplicon size and annealing temperature.

Gene	Oligonucleotides	Sequence (5′-3′)	Amplicon size (bp)	(°C)
SATB2	Forward; Reverse	TGTCTATCATGTTGTGACGTTGA; TCATCTCTTTGAGCAGTTCCTTTA	150	63
EWS-R1	Forward; Reverse	GCAACAAAGCTATGGAACCTATG; GGGCAGTGGAGTAGTAAACC	137	62
Nanog	Forward; Reverse	CCCAGCTGTGTGTACTCAAT; GGTTCAGGATGTTGGAGAGTT	87	60
POU5F1	Forward; Reverse	GGGAGGAGCTAGGGAAAGA; TCCTTCCTTAGTGAATGAAGAACT	77	60
Sox2	Forward; Reverse	TGCAGTACAACTCCATGA; GGACTTGACCACCGAACC	125	55
KLF4	Forward; Reverse	CGGGAAGGGAGAAGACACT; AGTCGCTTCATG TGGGAGA	79	60
LIN28A	Forward; Reverse	CGACTGTAAGTGGTTCAAC; CCTTCCATGTGCAGCTTACT	100	60
MYC	Forward; Reverse	GCTGCTTAGACGCTGGATTTTT; GAGTCGTAGTCGAGGCATAGT	110	63
PROM1	Forward; Reverse	CCAGAAGCCGGGTCAAAAT; ATTCACTCAAGGCACCATCC	127	60
ALDH1A1	Forward; Reverse	TGATTCAGTGAGTGGCAAGAA; CCTGCAACATCCTCCTTATCT	98	60
CD34	Forward; Reverse	GTACCCTTGGAAGTACCAGCCT; CAGAGGTAGATGTGAATTTGAC	100	60
EZR	Forward; Reverse	GCCTTCTTGTCGATGGGTTA; GCCTCTTGTCGATGGGTTTA	134	61
AXL	Forward; Reverse	TTAGTGCTACGCGGAATGG; CCTATGTCCATAGCACCTCG	133	60
PPARγ	Forward; Reverse	GTCGGTTTCAGAAATGCCTTG; ATCTCCGCCAACAGCTTC	97	57
LPL	Forward; Reverse	TGCATTTCAATCACAGCAGCAA; TACAGGGCGGCCACAAG	101	57