The Regulatory Effects of Long Noncoding RNA-ANCR on Dental Tissue-Derived Stem Cells

Figure 3

The effect of ANCR-RNAi on osteogenic differentiation in DTSCs. ALP activity was significantly increased in DPSC/ANCR-RNAi (a), PDLSC/ANCR-RNAi (b), and SCAP/ANCR-RNAi (c) cells when compared with control cells. Three weeks after induction, the cells were stained with Alizarin red (pH = 4.1), and DPSC/ANCR-RNAi (d), PDLSC/ANCR-RNAi (e), and SCAP/ANCR-RNAi (f) cells formed more mineralized nodules than the control groups. The Alizarin red quantification confirmed this result. The mRNA expression of ALP, BSP, and OCN was upregulated in DPSC/ANCR-RNAi (g), PDLSC/ANCR-RNAi (h), and SCAP/ANCR-RNAi (i) cells compared with control cells after two weeks of induction. when compared with the control group.