High-Fidelity Reprogrammed Human IPSCs Have a High Efficacy of DNA Repair and Resemble hESCs in Their MYC Transcriptional Signature

Figure 3

hESCs and hiPSCs have similar kinetics of apoptotic response to radiation exposure. ((a)(i), (a)(ii), and (b)) Representative Western blot analysis depicting the expression of p53 and PARP1 (full-length: 116 kDa; cleaved form: 89 kDa) in cell lysates from H9, ES03, CB6.2, iCB9, and iHuF3 at time 0 h and at 1 h, 2 h, and 4 h after IR. (c–e) Densitometry analysis of the western blots for measurement of (c) total p53, (d) phosphorylated (p-p53), and (e) PARP1 cleavage, using ImageJ software. Statistical significance of the data was determined using 2-way ANOVA with Bonferroni posttests to compare the replicates (three independent experiments).

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