Research Article
PS1/γ-Secretase-Mediated Cadherin Cleavage Induces β-Catenin Nuclear Translocation and Osteogenic Differentiation of Human Bone Marrow Stromal Cells
Figure 3
Expression of E-cadherin, N-cadherin, and β-catenin in osteoinduced and Dapt-treated BMSCs. Confocal microscopy images showing the expression of E-cadherin ((a) red), N-cadherin ((b) red), β-catenin ((c) green), and Pan-cadherin ((d) green) by BMSCs cultured for 48 h with expansion medium (CT, control undifferentiated), osteogenic medium (OM), or osteogenic medium containing 20 μM of the PS1/γ-secretase inhibitor Dapt (Dapt + OM). Dapt was added 24 h before the addition of OM. Nuclei were stained with DAPI (blue). Scale bars = 25 μm.
(a) |
(b) |
(c) |
(d) |