PS1/γ-Secretase-Mediated Cadherin Cleavage Induces β-Catenin Nuclear Translocation and Osteogenic Differentiation of Human Bone Marrow Stromal Cells

Figure 5

PS1/γ-secretase inhibition impairs β-catenin nuclear translocation and signaling. (a) Representative immunoblotting of total β-catenin in whole protein extracts. (b) Densitometry of total β-catenin bands obtained in 5 independent experiments. Data represent mean ± SD. (c) Representative immnunoblotting of the active form of β-catenin (ABC) in cytoplasmic and nuclear cell fractions from two different donors. (d) Densitometry of ABC nuclear bands normalized by lamin A. (e) Luciferase activity in BMSCs containing a SuperTop TCF reporter, osteoinduced for 2 and 5 days in the presence of Dapt. Bars represent mean ± SD of 5 different donors performed in triplicate. CT = control undifferentiated cells; OM = osteogenic medium; Dapt + OM = osteogenic medium with Dapt. .

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