Lipogems Product Treatment Increases the Proliferation Rate of Human Tendon Stem Cells without Affecting Their Stemness and Differentiation Capability

Figure 1

Schematic illustration of the Lipogems device and the transwell coculture system of hTSCs with the Lipogems product. The lipoaspirate was processed using the Lipogems device to obtain the final Lipogems product. A transwell coculture system was used to study the effects of the Lipogems product on primary cultures of hTSCs. Cells were seeded on the bottom of a 6-well plate in normal growth medium. Twenty-four hours after plating, the Lipogems product was transferred in the upper well of a transwell cell culture system (250 μL/transwell insert), separated from hTSCs in the lower well by a 0.4 μm microporous polycarbonate membrane. hTSCs and Lipogems product were maintained in coculture for successive analyses.