Evaluating the Impact of Human Amnion Epithelial Cells on Angiogenesis

Table 2

Primer sequences and annealing temperatures.

Gene	Primer sequence	Annealing temperature
VEGFA	Fwd: CTACCTCCACCATGCCAAGTG	60°C
	Rev: TGATTCTGCCCTCCTCCTTCT
PDGFB	Fwd: AATGGTCACCCGAGTTTGG	60°C
	Rev: CTGGCATGCAAGTGTGAGAC
ANGPT1	Fwd: CCTGATCTTACACGGTGCTGATT	60°C
	Rev: GTCCCGCAGTATAGAACATTCCA
VEGFR1	Fwd: CGGGGATTTCACTGTACATCT	60°C
	Rev: AAGCAAACCACACTGGCTTC
VEGFR2	Fwd: CCCTGCCGTGTTGAAGAGTT	60°C
	Rev: GGACAGGGGGAAGAACAAAA
PDGFR	Fwd: AGCTGGCAGAGGATTAGGCT	60°C
	Rev: CTCCATGTGTGGGACATTCA
PDGFR	Fwd: CAGGAGAGACAGCAACAGCA	60°C
	Rev: TGTCCAGAGCCTGGAACTGT
Tie-2	Fwd: AGTCTTATGTGTTCTGTCTCCCTGACC	60°C
	Rev: TCATCCTCGGTATGCCTTCTCTCTCAC
FOXC1	Fwd: ACCTTGACGAAGCACTCGTT	60°C
	Rev: CGGCATCTACCAGTTCATCA