Theoretical and Practical Issues That Are Relevant When Scaling Up hMSC Microcarrier Production Processes

Figure 2

Microcarrier-cell-aggregate formation during the cultivation of the hASCs. (a) Time-dependent profiles of the Sauter mean diameters for all tested impeller speeds. (b) and (c) DAPI staining picture of microcarrier-cell-aggregate sample taken from a run at suspension criterion (49 rpm, day 7). White arrow indicates that the cell growth takes place over the entire microcarrier surface and between the microcarriers. Focal plane was set in order to see the cell growth between the microcarriers.

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