Tissue Inhibitor of Matrix Metalloproteinases-1 Knockdown Suppresses the Proliferation of Human Adipose-Derived Stem Cells

Figure 2

TIMP-1 expression was suppressed by shRNA-TIMP-1. TIMP-1 expression was analyzed by real-time RT-PCR and western blotting, respectively. (a) The levels of TIMP-1 mRNA in parental ADSCs, mock, and shTIMP-1 ADSCs were measured by real-time RT-PCR 72 h after shRNA infection. TIMP-1 mRNA expression in parental ADSCs is defined as 1. The -axis represents the normalized TIMP-1 mRNA expression relative to parental ADSCs. (b) Representative western blot images for the detection of TIMP-1 protein expression in the ADSCs infected with shRNA against TIMP-1. (c) The relative expression level of TIMP-1 protein was quantified by the ratio of optical density of TIMP-1 and β-actin. The data are denoted as mean ± SD (). versus mock-shRNA group. Parental ADSCs: uninfected ADSCs; mock ADSCS: control lentivirus infected ADSCs; shTIMP-1 ADSCs: shRNA-TIMP-1 lentivirus infected ADSCs.

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