Tissue Inhibitor of Matrix Metalloproteinases-1 Knockdown Suppresses the Proliferation of Human Adipose-Derived Stem Cells

Figure 4

Immunocytochemical staining of Ki67. Ki67 staining was performed to evaluate cell proliferation after TIMP-1 knockdown in the ADSCs. (a) Representative images of Ki67 staining in the parental ADSCs, mock-shRNA ADSCs, and shTIMP-1 ADSCs. (b) Percentage of Ki67 positive cells was quantified in four random areas as described in Section 2. The data are denoted as mean ± SD (). versus mock-shRNA groups. Scale bar: 100 μm.

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