Adipose Derived-Mesenchymal Stem Cells Viability and Differentiating Features for Orthopaedic Reparative Applications: Banking of Adipose Tissue

Figure 6

Cryopreservation at −80°C and −196°C maintained morphology of adipose tissue and the content of ATD-MSCs. The morphology of cryopreserved adipose tissue at −80°C and −196°C was maintained, without evidence of tissue degeneration or necrosis, comparable to the fresh sample (a–c). The percentage of CD105, CD44, and CD73+ ATD-MSCs and the CD271+ subpopulation was comparable to fresh samples and also between the two temperatures (d, e).

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