Isolation, Characterization, and Multipotent Differentiation of Mesenchymal Stem Cells Derived from Meniscal Debris

Table 1

Primer sequences for quantitative real-time PCR.

Target gene	Primer sequence (5′-3′)
	Forward	Reverse
Runx2	TGGTTACTGTCATGGCGGGTA	TCTCAGATCGTTGAACCTTGCTA
ALP	ACCACCACGAGAGTGAACCA	CGTTGTCTGAGTACCAGTCCC
OCN	GGCGCTACCTGTATCAATGG	GTGGTCAGCCAACTCGTCA
Collagen I	GAGGGCCAAGACGAAGACATC	CAGATCACGTCATCGCACAAC
PPARγ	GATGCCAGCGACTTTGACTC	ACCCACGTCATCTTCAGGGA
Adiponectin	GGTGCTGAAGCCTACCAAC	AGGAAGAACAGACGGCAGAAC
LPL	TCATTCCCGGAGTAGCAGAGT	GGCCACAAGTTTTGGCACC
PAS	AAGGACCTGTCTAGGTTTGATGC	TGGCTTCATAGGTGACTTCCA
aP2	ACTGGGCCAGGAATTTGACG	CTCGTGGAAGTGACGCCTT
SOX9	AGCGAACGCACATCAAGAC	CTGTAGGCGATCTGTTGGGG
Collagen II	TGGACGCCATGAAGGTTTTCT	TGGGAGCCAGATTGTCATCTC
GAG	ACTCTGGGTTTTCGTGACTCT	ACACTCAGCGAGTTGTCATGG
GAPDH	ACACTCAGCGAGTTGTCATGG	ACACCATGTATTCCGGGTCAAT