Attachment, Growth, and Detachment of Human Mesenchymal Stem Cells in a Chemically Defined Medium

Table 1

Properties of the enzymes used for cell detachment.

Detachment enzyme	Manufacturer	Incubation time (min)
Accutase	Sigma-Aldrich	10
Collagenase	PAA Laboratories GmbH	60
Prolyl-specific peptidase (PsP)	[23]	40
Trypsin	PAA	10