Potential Role of Activating Transcription Factor 5 during Osteogenesis

Figure 1

(a) Alizarin Red S staining of hADSCs during osteogenic differentiation. Mineralization of the extracellular matrix with the presence of calcium precipitates was visualized by staining with Alizarin Red S at days 8, 16, and 24. (b) Gene expression levels during osteogenic differentiation of hADSCs. Relative quantification of mRNA level assessed by qRT-PCR for RUNX2, OSTERIX, and ALPL after 3, 11, 16, 21, and 24 days of osteogenic differentiation. Proliferation group is used as reference for quantitation.

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