Biological Effects of Culture Substrates on Human Pluripotent Stem Cells

Table 1

Summary of culture substrates used for culturing undifferentiated hPSCs.

Name	Commercial name (if any, only major)	Material type	References
Gelatinous protein mixture	Matrigel (Corning)	Crude extract secreted by EHS mouse sarcoma cells	[9, 28]
	Geltrex (Thermo Fisher Scientific)
	Cultrex BME (Trevigen)
Laminin		Extracted protein	[9]
Laminin-511	LN511 (Biolamina)	Recombinant protein	[29, 30]
Laminin-521	LN521 (Biolamina)	Recombinant protein	[31, 32]
	rhLaminin-521 (Thermo Fisher Scientific)
Laminin-E8 fragment	iMatrix-511 (Nippi)	Recombinant protein	[33, 34]
Vitronectin	Vitronectin XF (Stem Cell Technologies)	Extracted or recombinant protein	[35–38]
Truncated vitronectin	VTN-N (Thermo Fisher Scientific)	Recombinant protein	[39]
Fibronectin		Extracted protein	[40–42]
Collagen type I		Extracted protein	[20, 43]
Nanofibrous gelatin		Processed gelatin	[44]
Customized spider silk protein		Recombinant protein, containing vitronectin motif	[45]
Peptide-acrylate surfaces (PAS)	Synthemax (Corning)	Synthetic polymers with peptides	[46]
PMEDSAH		Synthetic polymer	[47]
Synthetic substrates displaying heparin-binding peptides		Synthetic polymers with peptides	[48]
Polyvinylalcohol-co-itaconic acid hydrogels grafted with aoligopeptide derived from vitronectin (KGGPQVTRGDVFTMP)		Synthetic polymers with peptides	[49]
UV/ozone radiation		Modification of typical cell culture plastics	[50]
Hydrogel interfaces of aminopropylmethacrylamide (APMAAm)		Synthetic polymers	[51]
Human E-cadherin-Fc chimeric protein		Recombinant protein	[31, 52]