A Systematic Comparison Identifies an ATP-Based Viability Assay as Most Suitable Read-Out for Drug Screening in Glioma Stem-Like Cells

Figure 2

Comparison of all tested viability assays. U373 cells (left column) or T98 cells (right column) were treated with (a) radiation (3 Gy (white bars), 6 Gy (black bars)), (b) TMZ (10 μM (white bars), 50 μM (grey bars), and 100 μM (black bars)), (c) rapamycin (10 nM (white bars), 50 nM (grey bars), and 100 nM (black bars)), or (d) Delta24-RGD (MOI 10 (white bars), MOI 30 (grey bars), and MOI 100 (black bars)). Read-out was after 5 or 6 days with the WST-1 assay (WST), CellTiter-GLO assay (CTG), CellTiter-Fluor assay (CTF), Alamar Blue assay with absorbance (ABa), Alamar Blue assay with fluorescence (ABf), crystal violet (CV) assay, or IncuCyte (IC). Results are expressed as mean percentage of viability compared to nontreated controls (100%, dotted line). Error bars indicate standard deviation, .

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