Chemokine (C-X-C) Ligand 12 Facilitates Trafficking of Donor Spermatogonial Stem Cells

Figure 4

CXCL12/CXCR4 pathway promotes germ cell migration. SSC-enriched germ cells seeded in upper transwell in the presence or absence of AMD3100 (1.25 μM) were exposed to CXCL12 (10 ng/mL) in the lower well. Measurement of the absorbance (562 nm) in the bottom well was used to assess the active number of germ cells that migrated from upper to lower wells. Data are means ± SEM, where significance was assessed using a Student’s -test (, experimental replicates).