Validation of Housekeeping Genes to Study Human Gingival Stem Cells and Their In Vitro Osteogenic Differentiation Using Real-Time RT-qPCR

Table 2

The 4 algorithms used in the study.

Analysis method	PCR values	Stability values	Type of analysis
The ΔCt comparative method	Ct	StdDev mean	Intergene
GeNorm		, NF	Intergene; One HKG all other HKGs; Stable HKGs: lowest variability: low
BestKeeper	Ct	SD,	Intra- and intergene; Pairwise correlation analysis BestKeeper index; Stable HKGs: low SD and high
NormFinder			Intra- and intergene; stable HKGs: least intra- and intergroup variations